Therefore, we compared the gene expression profile of 5-AZAdC-treated and untreated GC cell lines by a microarray assay. Epigenetic manipulation of GC cell lines is a useful tool to better understand gene expression regulatory mechanisms for clinical applications. The use of 5-Aza-2′-deoxycytidine (5-AZAdC) was approved for the treatment of myelodysplastic syndromes, and this drug can treat solid tumours at low doses. Very few therapeutic options are currently available in this neoplasia. Gastric cancer (GC) is the third leading cause of cancer-related death worldwide. EPIC-TABSAT is freely accessible to all users at. Together with the computation of target-specific epialleles it is useful in validation, research, and routine diagnostic environments.
The graphical user interface offers an unprecedented way to interpret TBS data alone or in combination with array-based methylation studies. The tool can handle multiple targets as well as multiple sequencing files in parallel and covers the complete data analysis workflow from calculation of quality metrics to methylation calling and interactive result presentation. Consequently, we have developed EPIC-TABSAT, a user-friendly web-based application for the analysis of targeted sequencing data that additionally allows the integration of array-based methylation results. Yet, an easy-to-use tool for the analysis of TBS data in combination with array-based methylation results has been missing. In addition to chip and sequencing based epigenome wide methylation profiling methods, targeted bisulfite sequencing (TBS) has been established as a cost-effective approach for routine diagnostics and target validation applications. DNA methylation is one of the major epigenetic modifications and has frequently demonstrated its suitability as diagnostic and prognostic biomarker.
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