cumini on blood coagulation and anticoagulation factors in healthy white albino rabbits at different doses.īlood samples were drawn twice during this study and biochemical assays were performed to determine the effect on different parameters such as coagulation, anticoagulation, hematological, Protein C (PC) and thrombin antithrombin (TAT) complex and platelet aggregation. Therefore, aim of this present investigation is to examine the effects of methanolic extract of S. cumini on blood coagulation and other parameters of blood were poorly pharmacologically studied.
is one of the very popular traditionally used medicinal plants with numerous pharmacological activities including antioxidant, hypoglycemic and anti-inflammatory.
The results of the present study established Jamun honey as good as Manuka honey considering wound closure, re-epithelialization, collagen deposition and pro-angiogenic potential. All these observations depicted that JH promotes sequential stages of wound healing in diabetic mice model. It also modulated vital angiogenic markers (viz HIF-1α, VEGF, VEGF R–II) significantly (*p ≤ 0.05).
Topical application of JH in diabetic mice model showed significant (*p ≤ 0.05) wound closure, reepithelialization, collagen deposition (I/III) and balanced the myofibroblasts formation. JH (0.1% v/v) dilution has notably improved wound closure, migration with concomitant α-SMA expressions in vitro. We applied FDA approved Manuka honey (MH) as positive control and JH as test honey to evaluate wound re-epithelialization, sub-epithelial connective tissue modification and angiogenesis via histo-pathological and immuno-histochemical analysis. The fibroblast cell model was studied for migratory behaviour and myofibrolasts infiltration under honey interventions via scratch/migration assay, immuno-cytochemistry and western blot.
So present study was designed to evaluate the efficacy of Jamun honey (JH) for diabetic wound healing in in vitro wound (primary fibroblasts) model and in in vivo of diabetic mice (Streptozotocin induced) model. Jamun (Syzygium cumini) honey may be a promising candidate for diabetic wound healing and need to explore in detail. Despite of meaningful progress in diabetic treatment, management of diabetic chronic wounds is still challenging. Diabetic patients are frequently afflicted with impaired wound healing where linear progression of molecular and cellular events compromised.
0 kommentar(er)
